The Salimetrics Research Library
Look up current saliva research on
Cotinine
and related analytes here!
Cotinine
When nicotine from tobacco smoke is taken into the lungs and enters the bloodstream, the principle metabolite produced in the liver is cotinine. Cotinine diffuses easily from blood into saliva, and salivary and blood levels are highly correlated. (1) Cotinine in saliva has a longer half-life than nicotine (more than 10 hours), and the literature has documented it to be a specific and sensitive marker for determining exposure to tobacco and nicotine. (2,3,4) More »
References
- Benowitz, N.L. (1996). Cotinine as a biomarker of environmental tobacco smoke exposure. Epidemiol Rev, 18(2), 188-204.
- Dhar, P. (2004). Measuring tobacco smoke exposure: Quantifying nicotine/cotinine concentration in biological samples by colorimetry, chromatography and immunoassay methods. J Pharm Biomed Anal, 35(1), 155- 68.
- Alterman, A.I., Gariti, P., Niedbala, R.S. (2002). Varying results for immunoassay screening kits for cotinine levels. Psychol Addict Behav, 16(3), 256-59.
- Van Vunakis, H., Tashkin, D.P., Rigas, B., et al. (1989). Relative sensitivity and specificity of salivary and serum cotinine in identifying tobacco-smoking status of self-reported non-smokers and smokers of tobacco and/or marijuana. Arch Environ Health, 44(1), 53-58.
Cotinine Overview
| Approved Salivary Collection Devices | Serum-Saliva Correlation | Sensitivity | Sample Test Volume | Recommended Collection Volume | Special Considerations | Related Analytes |
|---|---|---|---|---|---|---|
| No | 0.15 ng/mL | 20 μL | 75 μL* | No | No |
*In addition to the volume recommended for each analyte, we recommend collecting an additional 300 μL to allow for liquid handling loss and possible repeat tests (500 µL recommended for TNF-α and IL-1β).
Salimetrics Products and Services for Cotinine
| Testing Service | DNA Panel Available | Research Assay Kit | IVD Assay Kit | CE Marked Kit | Collection Supplies | Technical Support |
|---|---|---|---|---|---|---|
| Yes | Yes | Yes Specifications» |
Yes Specifications» |
Yes Specifications» |
Yes | Yes |
Salivary Cotinine Kit Specifications
| Target Analyte: | Cotinine |
| Catalog number: | 1-2002 (5PK 1-2002-5) |
| Testing protocol: | Download PDF |
| Format: | 96-well plate |
| Type: | Quantitation |
| Method: | EIA |
| Calibrator range: | 0.8 ng/mL - 200 ng/mL |
| Sensitivity: | 0.15 ng/mL |
| Saliva volume/test: | 20 µL |
| Incubation time: | 2.0 hours |
| Tests per kit: | 76 (singlet) |
| Correlation with serum: | N/A |
| Controls included in kit? | Yes |
| MSDS sheets: | Available upon request |
Salivary Cotinine CE Marked Kit Specifications
| Target Analyte: | Cotinine |
| Catalog number: | 1-2112 (5-pack 1-2112-5) |
| Testing protocol: | Download PDF |
| Format: | 96-well plate |
| Type: | Quantitation |
| Method: | EIA |
| Calibrator range: | 0.8 ng/mL - 200 ng/mL |
| Sensitivity: | 0.15 ng/mL |
| Saliva volume/test: | 20 µL |
| Incubation time: | 2.0 hours |
| Tests per kit: | 76 (singlet) |
| Correlation with serum: | N/A |
| Controls included in kit? | Yes |
| MSDS sheets: | Available upon request |
Salivary Cotinine IVD Assay Kit Specifications
| Target Analyte: | Cotinine |
| Catalog number: | 1-2112 (5-pack 1-2112-5) |
| Testing protocol: | Download PDF |
| Format: | 96-well plate |
| Type: | Quantitation |
| Method: | EIA |
| Calibrator range: | 0.8 ng/mL - 200 ng/mL |
| Sensitivity: | 0.15 ng/mL |
| Saliva volume/test: | 20 µL |
| Incubation time: | 2.0 hours |
| Tests per kit: | 76 (singlet) |
| Correlation with serum: | N/A |
| Controls included in kit? | Yes |
| MSDS sheets: | Available upon request |
