The Salimetrics Research Library
Look up current saliva research on
Estrone
and related analytes here!
Estrone
Estrone (3-hydroxy-1,3,5(10)-estratrien-17-one; E1) is one of the three main estrogenic steroid hormones produced in humans. Circulating estrone levels are relatively high at birth in both males and females, decrease postnatally, and increase during puberty. (1) Of the three major estrogens, estrone is predominant after menopause in women. (1)
In premenopausal women estrone is primarily produced by conversion of androstenedione in the ovaries, with concentrations peaking in the preovulatory phase and a smaller secondary increase during the luteal phase. (1,2) In post-menopausal women, children, and men, estrone is largely produced by conversion of androstenedione in peripheral tissues. (1,2,3) Estrone is subsequently reduced to estradiol in various peripheral tissues. (3)
In non-pregnant women, only about 3% of estrone in the bloodstream is not bound to proteins. (4) Unbound estrone enters saliva from blood via intracellular mechanisms, and in saliva the majority of the estrone remains unbound to protein. The saliva and plasma values are highly correlated. (5) More »
References
- Faiman, C., Winter, J.S., Reyes, F.I. (1976). Patterns of gonadotrophins and gonadal steroids throughout life. Clin Obstet Gynecol, 3(3), 467-83.
- Cañez, M.S., Lee, K.J., & Olive, D.L. (1992). Progestogens and estrogens. Infert Reprod Med Clin North Amer, 3, 59-78.
- Sasano, H., Harada, N. (1998). Intratumoral aromatase in human breast, endometrial, and ovarian malignancies. Endocr Rev, 19(5), 593-607.
- Speight, A.C., Hancock, K.W., Oakey, R.E. (1979). Non-protein-bound oestrogens in plasma and urinary excretion of unconjugated oestrogens in non-pregnant women. J Endocrinol, 83, 385-91.
- Vining, R.F., McGinley, R.A. (1987). The measurement of hormones in saliva: Possibilities and pitfalls. J Steroid Biochem, 27(1-3), 81-94.
Estrone Overview
| Approved Salivary Collection Devices | Serum-Saliva Correlation | Sensitivity | Sample Test Volume | Recommended Collection Volume | Special Considerations | Related Analytes |
|---|---|---|---|---|---|---|
| No | 1 pg/mL | 100 μL | 225 μL* | No |
*In addition to the volume recommended for each analyte, we recommend collecting an additional 300 μL to allow for liquid handling loss and possible repeat tests (500 µl recommended for TNF-α and IL-1β).
Salimetrics Products and Services for Estrone
| Testing Service | DNA Panel Available | Research Assay Kit | IVD Assay Kit | CE Marked Kit | Collection Supplies | Technical Support |
|---|---|---|---|---|---|---|
| Yes | Yes | Yes Specifications» |
No | No | Yes | Yes |
Salivary Estrone Kit Specifications
| Target Analyte: | Estrone |
| Catalog number: | 1-3202 (5PK 1-3202-5) |
| Testing protocol: | Download PDF |
| Format: | 96-well plate |
| Type: | Quantitation |
| Method: | EIA |
| Calibrator range: | 3.1 pg/mL - 300 pg/mL |
| Sensitivity: | 1 pg/mL |
| Saliva volume/test: | 100 µL |
| Incubation time: | 3 hours |
| Tests per kit: | 76 (singlet) |
| Correlation with serum: | N/A |
| Controls included in kit? | Yes |
| MSDS sheets: | Available upon request |
