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Change to estradiol diluent in the Estradiol HS EIA kit

06.01.09

The estradiol diluent in the Salimetrics Estradiol HS EIA kit (research use #1-3702/1-3702-5 and diagnostic use #1-4702/1-4702-5) has been changed to improve sensitivity of the assay. No changes have been made to any other reagents, assay protocol, performance or specificity. The release date for the kit with the new estradiol diluent will be June 1st, 2009.

Saliva assayed for estradiol using OLD diluent and NEW diluent correlate very highly [r(54) = 0.99, p<0.001]. As anticipated, there is a shift to lower expected MEAN values.

OLD diluent NEW diluent
Sensitivity = <1 pg/mL Sensitivity = 0.1 pg/mL
Mean = 12.63 pg/mL Mean = 9.55 pg/mL
SD = 6.61 pg/mL SD = 5.35 pg/mL

To facilitate your data transition process, we are providing the following conversion formulas:

  1. Correlating OLD diluent data to NEW diluent data:
    (OLD value x 0.8014) – 0.5736 = NEW value
  2. Correlating NEW diluent data to OLD diluent data:
    (NEW value x 1.22) + 0.956 = OLD value

*Salivary Estradiol Expected Ranges with NEW diluent

Changes to normal ranges should be noted on laboratory reports to notify physicians.

Pre-menopausal Adult Women N Mean (pg/mL) Standard Deviation
(pg/mL)
Follicular 20 1.35 0.80
Mid-cycle 19 2.97 1.58
Luteal 20 2.56 0.84

*To be used as a guide only. Each laboratory should establish new normal ranges. Any quantitative results indicating abnormal estradiol levels should be evaluated along with patient history, symptoms and other laboratory tests.

Please contact the Salimetrics Technical Service Team with any questions.
Email: support@salimetrics.com
Tel: 1-800-790-2258