Our new Cotinine EIA Kit is an efficient, highly accurate, and cost-effective means of quantitatively monitoring nicotine exposure. The kit is designed specifically for studying the use of tobacco (or nicotine products) in population-based and national surveys,
monitoring compliance with smoking cessation and treatment intervention programs, estimating the effects of second-hand nicotine exposure, or screening employees for health risk.
The assay is exceptionally sensitive, 0.05ng/ml, making it ideal for use in the study of exposure of children to secondhand smoke, or of adults to smoke in public or workplace environments.
The test volume of saliva is minute, only 20µl, and can be collected in only two minutes. Collection is noninvasive, and can be accomplished using a variety of collection devices (Sorbette, Salivette, and passive drool). Research subjects can easily collect the samples themselves in the home or workplace without the aid of a technician.
Laboratories testing employees for health risk will find the short incubation time of 2.0 hours cost-effective and time-efficient. The assay protocol is highly reliable and will reduce the number of samples requiring repeated testing.
Cross-reactivity of the antibody with nicotine is 1.49 percent, less than 0.1 percent with nicotinic acid and nicotinamide, and 55% with 3-OH cotinine (metabolite of cotinine).
U.S. customers: Please ask for Catalog No. 1-2002. International customers should request Catalog No. 1-2012.
New Salivary Enzyme Immunoassay Quantitatively Measures Cotinine, A Metabolite of Nicotine
Table 1: Salivary cotinine levels (ng/ml) in smoking and nonsmoking mothers and their six month-old infants.
Salimetrics' quantitative enzyme immunoassay for salivary cotinine discriminates smokers from non-smokers, and differentiates primary- from secondary-smoke exposure
Mother's Self-Reported Status: Smokers (n=27)
Group
Mean
Standard
Deviation
Number of cigarettes smoked in
prior 48 hours by mother
Mother's salivary cotinine (ng/ml)
Infant salivary cotinine (ng/ml)
14.67
176.67
8.58
14.26
241.27
9.59
3-60
0-565.36
0.91-33.19
Ranges
Mother's Self-Reported Status: Non-smokers (n=20)
Group
Mean
Standard
Deviation
Number of cigarettes smoked in
prior 48 hours by mother
Mother's salivary cotinine (ng/ml)
Infant salivary cotinine (ng/ml)
NA
5.45
3.54
0.0
2.15
2.12
Ranges
NA
0-23.9
0-12.34
Notes:
1. Smoking status determined by number of cigarettes smoked in the past 48 hours, “0” = non-smoker, “> 3” smoker.
2. Independent sample t-test comparing smoking and non-smoking groups, p < 0.001.
Data from: Granger, D. A., Blair, C ., Willoughby , M., Kivlighan, K. T. and The Family Life Project Investigators (2005). Salivary cotinine levels in mothers and their six-month old infants. Unpublished Technical Report.
Figure 1. Positive association between mother and infant salivary cotinine (ng/ml)
Figure 2. Positive association between number of cigarettes smoked in the past 48 hours and salivary cotinine (ng/ml) in mothers
Copyright 2005 Salimetrics LLC