Cortisol Saliva Collection
Bovine hormones normally present in dairy products can cross-react with anti-cortisol antibodies and cause false results.
Hormone based oral contraceptives and estrogens, may potentially interfere with cortisol levels in saliva
Some steroid-based anti-inflammatory medications and multiple medications mimic the structure of cortisol and have the potential to influence cortisol levels in saliva
Saliva Collection Protocol
Before Sample Collection
- Avoid foods with high sugar, acidity, or caffeine immediately before sample collection.
- Document consumption of alcohol, caffeine, nicotine, and prescription/over-the-counter medications within the prior 12 hours.
- Document vigorous physical activity and the presence of oral disease, injury or inflammation.
- Do not brush teeth or eat a major meal within 60 minutes of sample collection.
- Rinse mouth with water to remove food residue and then wait at least 10 minutes before collecting saliva (rinsing is not recommended for awakening sample).
During Sample Collection
- Recommended Collection Volume: 75 µl*
- Use a collection device that has been validated for the measurement of this analyte.
- Follow your selected sample collection device/method protocol.
*Add 300 µl to the total volume of all tests for liquid handling loss
After Sample Collection
- Record the time and date of specimen collection.
- Refrigerate samples immediately (if possible) and freeze at or below -20°C (household freezer) as soon as possible (within 6 hours of sample collection)
- Samples visibly contaminated with blood should be recollected.
- Do not add preservatives to saliva samples unless it has been previously validated with the assay.