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Cortisol Saliva Collection

Approved Collection Methods

Passive Drool

SalivaBio Swabs

Special Considerations

Bovine hormones normally present in dairy products can cross-react with anti-cortisol antibodies and cause false results.

Hormone based oral contraceptives and estrogens, may potentially interfere with cortisol levels in saliva

Some steroid-based anti-inflammatory medications and multiple medications mimic the structure of cortisol and have the potential to influence cortisol levels in saliva

Saliva Collection Protocol

Before Sample Collection

  • Avoid foods with high sugar, acidity, or caffeine immediately before sample collection.
  • Document consumption of alcohol, caffeine, nicotine, and prescription/over-the-counter medications within the prior 12 hours.
  • Document vigorous physical activity and the presence of oral disease, injury or inflammation.
  • Do not brush teeth or eat a major meal within 60 minutes of sample collection.
  • Rinse mouth with water to remove food residue and then wait at least 10 minutes before collecting saliva (rinsing is not recommended for awakening sample).

During Sample Collection

  • Recommended Collection Volume: 75 µl*
  • Use a collection device that has been validated for the measurement of this analyte.
  • Follow your selected sample collection device/method protocol.

*Add 300 µl to the total volume of all tests for liquid handling loss

After Sample Collection

  • Record the time and date of specimen collection.
  • Refrigerate samples immediately (if possible) and freeze at or below -20°C (household freezer) as soon as possible (within 6 hours of sample collection)
  • Samples visibly contaminated with blood should be recollected.
  • Do not add preservatives to saliva samples unless it has been previously validated with the assay.