Technical Summary
| Analyte Summary | |
|---|---|
| Analyte: | Cotinine |
| Aliases: | Biomarker of Nicotine Exposure |
| Serum-Saliva Correlation: | NA |
| Optimum Collection Volume: | 75 μL* |
| Assay Summary | |
|---|---|
| Methodology: | ELISA |
| Sensitivity: | 0.15 ng/mL |
| Assay Range: | 0.8 ng/mL - 200 ng/mL |
| Assay Type: | Quantitative |
| Salivary Cotinine Example Ranges* | ||||
|---|---|---|---|---|
| Group | Number | Mean (ng/mL) | Standard Deviation (ng/mL) | Range (ng/mL) |
| Adult Smokers | 21 | 206.33 | 123.47 | 47.87-586.39 |
| Non-Smokers | 10 | 0 | 0 | NA |
Collect Saliva Samples
COTININE SALIVA COLLECTION CONSIDERATIONS
Better results begin with better saliva collection. This collection protocol features general considerations to maximize salivary cotinine analysis. Use this analyte-specific collection protocol to plan your collection methodology and sampling schemes.
APPROVED SALIVARY Cotinine COLLECTION METHODS
Test Saliva Samples
| Order Code (lab): | 5150-S |
| Transport Requirements: | Ship on Dry Ice |
Add DNA Analysis to My Study
Considerations for adding Salivary DNA to analyte Studies:
You can combine salivary analytes with easy, accurate, and affordable genomic testing using Salimetrics SalivaLab and the same sample that you are already collecting – no specialized saliva collection devices or additional samples are required.
Don’t know what SNPs are right for you? The SalivaLab’s DNA team specializes in genetic testing services, we recommend you Request a DNA Consult (gratis) to learn more about common considerations such as # of samples, participant ethnicity, and IRB Approval.
All DNA Services
DNA Extraction and Normalization
Single Nucleotide Polymorphism (SNP) Genotyping
VNTR & STR Analysis
References & Salivary Cotinine Research
- Benowitz, N.L. (1996). Cotinine as a biomarker of environmental tobacco smoke exposure. Epidemiol Rev, 18(2), 188-204.
- Dhar, P. (2004). Measuring tobacco smoke exposure: Quantifying nicotine/cotinine concentration in biological samples by colorimetry, chromatography and immunoassay methods. J Pharm Biomed Anal, 35(1), 155- 68.
- Alterman, A.I., Gariti, P., Niedbala, R.S. (2002). Varying results for immunoassay screening kits for cotinine levels. Psychol Addict Behav, 16(3), 256-59.
- Van Vunakis, H., Tashkin, D.P., Rigas, B., et al. (1989). Relative sensitivity and specificity of salivary and serum cotinine in identifying tobacco-smoking status of self-reported non-smokers and smokers of tobacco and/or marijuana. Arch Environ Health, 44(1), 53-58.
- Benowitz, N.L., Hukkanen, J., & Jacob, P., 3rd (2009). Nicotine chemistry, metabolism, kinetics and biomarkers. Handb Exp Pharmacol, 192, 29-60.
- Watts, R.R., Longone, J.J., Kinght, G.J., & Lewtas, J. (1990). Cotinine analytical workshop report: Consideration of analytical methods for determining cotinine in human body fluids as a measure of passive exposure to tobacco smoke. Environ Health Perspect, 84, 173-82.
- Roche, D., Callai, F., Reungoat, P., & Momas, I. (2001). Adaptation of an enzyme immunoassay to assess urinary cotinine in nonsmokers exposed to tobacco smoke. Clin Chem, 47(5), 950-52.
- Chard, T. (1990). An introduction to radioimmunoassay and related techniques (4th ed.). Amsterdam: Elsevier.
- Granger, D.A., Blair, C., Willoughby, M., Kivlighan, K.T., Hibel, L.C., Fortunato, C.K., Wiegand, L.E., & The Family Life Project Investigators (2007). Individual differences in salivary cortisol and alpha-amylase in mothers and infants: Relation to tobacco smoke exposure. Dev Psychobiol, 49(7), 692-701.