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IL-8 Saliva Collection

Approved Collection Methods

Passive Drool

Special Considerations

Studies show that levels of IL-8 in the oral fluid of healthy individuals do not reflect the levels of IL-8 in circulation. Levels of IL-8 in saliva may only represent individual differences in the degree of inflammation in the oral mucosal immune compartment.

This analyte is sensitive to freeze-thaw degradation. Sample collection, storage, and handling should be carefully designed to minimize the impact of freeze-thaw cycles.

Consider documenting parameters to estimate saliva flow-rate (ie; time taken to collect and sample volume). Consistency in collection method is recommended to avoid introducing unsystematic error into your study data.

Saliva Collection Protocol

Before Sample Collection

  • Avoid foods with high sugar, acidity, or caffeine immediately before sample collection.
  • Document consumption of alcohol, caffeine, nicotine, and prescription/over-the-counter medications within the prior 12 hours.
  • Document vigorous physical activity and the presence of oral disease, injury or inflammation.
  • Do not brush teeth or eat a major meal within 60 minutes of sample collection.
  • Rinse mouth with water to remove food residue and then wait at least 10 minutes before collecting saliva.

During Sample Collection

  • Recommended Collection Volume: 100 µl (part of the cytokine panel)
  • Use a collection device that has been validated for the measurement of this analyte.
  • Follow your selected sample collection device/method protocol.

After Sample Collection

  • Record the time and date of specimen collection.
  • Refrigerate samples immediately (if possible) and freeze at or below -20°C (household freezer) as soon as possible (within 6 hours of sample collection)
  • Samples visibly contaminated with blood should be recollected.
  • Do not add preservatives to saliva samples unless it has been previously validated with the assay.
  • Consider aliqouting samples to avoid multiple freeze-thaws.