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Salivary DHEA ELISA Kit

Technical Summary

Assay Protocol
Rev. 04.13.16
Specifications
Catalog#: 1-1202
Format: 96-well plate
Assay Time: ~ 4 hrs
Sample Volume/Test: 50 µL
Sensitivity: 5 pg/mL
Assay Range: 10.2 pg/mL - 1000 pg/mL
Storage Requirements: 2-8°C
Tests Per Kit
Singlet: 76
Duplicate: 38
Target Analyte
Technical Documentation

Salivary DHEA Assay Kit Overview

Intended Use

The Salimetrics DHEA Enzyme Immunoassay Kit is a competitive immunoassay specifically designed and validated for the quantitative measurement of salivary Dehydroepiandrosterone (DHEA). It is not intended for diagnostic use. It is intended only for research use in humans and some animals. Salimetrics has not validated this kit for serum or plasma samples.

Introduction

DHEA is a major secretory product of the adrenal glands; it is co-released along with cortisol in response to ACTH from the pituitary gland. Circulating levels of DHEA decline rapidly after birth and remain low until about the age of 6-8, when they begin to rise. This prepubertal onset of adrenal production, known as the adrenarche, involves enzymatic changes within the adrenal gland. DHEA levels increase until the third decade of life, then they begin to decline again. Significant reductions occur after the age of 50. A major role of DHEA is to act as a circulating precursor for conversion to androgens and estrogens in tissues throughout the body, and it has also been associated with immune function. In addition, DHEA is produced directly in the nervous system, where it functions as a neuroactive and neuroprotective factor. In blood, only 1-10% of DHEA is in its unbound or biologically active form. The remaining DHEA is bound to serum proteins. Unbound DHEA enters the saliva via intracellular mechanisms; the majority of DHEA in saliva is non-protein bound. Salivary DHEA levels are unaffected by salivary flow rate or salivary enzymes. DHEA exhibits a diurnal rhythm similar to cortisol, with highest levels in the morning after awakening, followed by a decline throughout the afternoon and evening.

Salivary DHEA Assay Principle

This is a competitive immunoassay kit. DHEA in standards and samples compete with DHEA conjugated to horseradish peroxidase for the antibody binding sites on a microtitre plate. After incubation, unbound components are washed away. Bound DHEA Enzyme Conjugate is measured by the reaction of the horseradish peroxidase enzyme to the substrate tetramethylbenzidine (TMB). This reaction produces a blue color. A yellow color is formed after stopping the reaction with an acidic solution. The optical density is read on a standard plate reader at 450 nm. The amount of DHEA Enzyme Conjugate detected is inversely proportional to the amount of DHEA present in the sample.

References & Salivary DHEA Research

  1. Kroboth, P.D., Salek, F.S., Pittenger, A.L., et al. (1999).  DHEA and DHEA-S: A review.  J Clin Pharmacol, 39(4), 327-48.
  2. Labrie, F. (2010).  DHEA, important source of sex steroids in men and even more in women.  Prog Brain Res, 182, 97-148.
  3. Labrie, F., Bélanger, A., Cusan, L., Candas, B. (1997).  Physiological changes in dehydroepiandrosterone are not reflected by serum levels of active androgens and estrogens but of their metabolites: Intracrinology.  J Clin Endocrinol Metab, 82(8), 2403-9.
  4. Labrie, F., Luu-The, V. Bélanger, A., et al. (2005).  Is dehydroepiandrosterone a hormone? J Endocrinol, 187, 169-96.
  5. Maninger, N., Wolkowitz, O.M., Reus, V.I., et al. (2009). Neurobiological and neuropsychiatric effects of dehydroepiandrosterone (DHEA) and DHEA sulfate (DHEAS).  Front Neuroendocrinol, 30(1), 65-91.
  6. Dillon, J.S. (2005).  Dehydroepiandrosterone, dehydroepiandrosterone sulfate and related steroids: Their role in inflammatory, allergic and immunological disorders.  Curr Drug Targets Infl Allergy, 4(3), 377-85.
  7. Widstrom, R., Dillon, J.S. (2004).  Is there a receptor for dehydroepiandrosterone or dehydroepiandrosterone sulfate?  Semin Repro Med, 22(4), 289-98.
  8. Baulieu, E.-E., Robel, P. (1998). Dehydroepiandrosterone (DHEA) and dehydroepiandrosterone sulfate (DHEA) as neuroactive neurosteroids.  Proc Natl Acad Sci U S A, 95(8), 4089-91.
  9. Charalampopoulos, I., Alexaki, V.-I., Tsatsanis, C., et al. (2006). Neurosteroids as endogenous inhibitors of neuronal cell apoptosis in aging.  Ann N Y Acad Sci, 1088, 139-52.
  10. Izawa, S. Sugaya, N., Shirotsuki, K., et al. (2008).  Salivary dehydroepiandrosterone secretion in response to acute psychosocial stress and its correlations with biological and psychological changes.  Biol Psych, 79(3), 294-98.
  11. Goodyer, I.M., Herbert, J., Altham, P.M., et al. (1996). Adrenal secretion during major depression in 8- to 16-year-olds, I. Altered diurnal rhythms in salivary cortisol and dehydroepiandrosterone (DHEA) at presentation. Psychol Med, 26(2), 245-56.
  12. Young, A.H., Gallagher, P., Porter, R. (2002). Elevation of the cortisol-dehydroepiandrosterone ratio in drug-free depressed patients.  Am J Psychiatry, 159(7), 1237-39.
  13. Harris, D.S., Wolkowitz, O.M., Reus, V.I. (2001). Movement disorder, memory, psychiatric symptoms and serum DHEA levels in schizophrienic and schizoaffective patients.  World J Biol Psychiatry, 2(2), 99-102.
  14. Christeff, N., Gherbi, N., Mammes, O., et al. (1997). Serum cortisol and DHEA concentrations during HIV infection.  Psychoneuroendocrinology, 22 (Suppl. 1), S11-18.
  15. Rosenfeld, R.S., Rosenberg, B.J., Fukushima, D.K., Hellman, L. (1975).  24-Hour secretory pattern of dehydroisoandrosterone and dehydroisoandrosterone sulfate.       J Clin Endocrinol Metab, 40(5), 850-5.
  16. Longcope, C. (1995). Metabolism of dehydroepiandrosterone.  Ann N Y Acad Sci, 774, 143-48.
  17. Simon, J.A. (2002). Estrogen replacement therapy: Effect on the endogenous androgen milieu.  Fertil Steril, 77 (Suppl 4), S77-82.)
  18. Vining, R.F., McGinley, R.A., Symons, R.G. (1983). Hormones in saliva: Mode of entry and consequent implications for clinical interpretation.  Clin Chem, 29(10), 1752-56.
  19. Shirtcliff, E.A., Granger, D.A., Schwartz, E., Curran, M.J. (2001). Use of salivary biomarkers in biobehavioral research: Cotton-based sample collection methods can interfere with salivary immunoassay results.  Psychoneuroendocrinology, 26(2), 165-73.)