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Serum-Saliva Correlation NA
Sensitivity 43 pg/mL
Sample Test Volume 100 μL
Recommended Collection Volume 225 μL*
Special Considerations

Passive Drool Only

DHEA-S is Flow Rate Dependent (pg/mL)

Collection Protocol Download PDF


  1. Summarized in Whetzel, C.A., Klein, L.C. Measuring DHEA-S in saliva: Time of day differences and positive correlations between two different types of collection methods.  BMC Res Notes, 3:204.
  2. Krobath, P.D., Salek, F.S., Pittenger, A.L. et al. (1999).  DHEA and DHEA-S: A review.  J Clin Pharmacol 39(4), 327-48.
  3. Rosenfeld, R.S., Rosenberg, B.J., Fukushima, D.K., Hellman, L. (1975).  24-Hour secretory pattern of dehydroisoandrosterone and dehydroisoandrosterone sulfate.  J Clin Endocrinol Metab, 40(5), 850-55.
  4. Carlström, K., Karlsson, R., Von Schoultz, B. (2002).  Diurnal rhythm and effects of oral contraceptives on serum dehydroepiandrosterone sulfate (DHEAS) are related to alterations in serum albumin rather than to changes in adrenocortical steroid secretion.  Scan J Clin Lab Invest, 62(5), 361-68.
  5. Labrie, F., Bélanger, A., Cusan, L., Candas, B. (1997).  Physiological changes in dehydroepiandrosterone are not reflected by serum levels of active androgens and estrogens but of their metabolites: Intracrinology.  J Clin Endocrinol Metab, 82(8), 2403-9.
  6. Labrie, F., Luu-The, V. Bélanger, A., et al. (2005).  Is dehydroepiandrosterone a hormone? J Endocrinol, 187, 169-96.
  7. Charalampopoulos, I., Alexaki, V.-I., Tsatsanis, C., et al. (2006).  Neurosteroids as endogenous inhibitors of neuronal cell apoptosis in aging.  Ann N Y Acad Science, 1088, 138-52.
  8. Baulieu, E.-E., Robel, P. (1998). Dehydroepiandrosterone (DHEA) and dehydroepiandrosterone sulfate (DHEAS) as neuroactive neurosteroids.  Proc Natl Acad Sci U S A, 95(8), 4089-91.)
  9. Kellner, M., Muhtz, C., Peter, F., et al. (2010).  Increased DHEA and DHEA-S plasma levels in patients with post-traumatic stress disorder and a history of childhood abuse. J Psychiatr Res, 44(4), 215-9.
  10. Golubchik, P., Mozes, T., Maayan, R., Weizman, A. (2009).  Neurosteroid blood levels in delinquent adolescent boys with conduct disorder. Eur Neuropsychopharmacol, 19(1), 49-52.
  11. Azurmendi, A., Braza, F., Garcia, F. et al. (2006). Aggression, dominance and affiliation: Their relationships with androgen levels and intelligence in 5-year-old children. Horm Behav, 50(1), 132-40.
  12. MacLaughlin, B.W., Wang, D., Noone, A.-M., et al. (2010).  Stress biomarkers in medical students participating in a mind body medicine skills program.  eCAM, doi:10.1093/ecam/neq039.
  13. Wang, J.-S., Chen, S.-M., Lee, S.-P., et al. 2009).  Dehydroepiandrosterone sulfate linked to physiologic response against hot spring immersion. Steroids, 74(12), 945-49.
  14. Vining, R.F., McGinley, R.A., Symons, R.G. (1983).  Hormones in saliva: Mode of entry and consequent implications for clinical interpretation.  Clin Chem, 29(10), 1752-56.
  15. Konttinen, Y.T., Stegaev, V., Mackiewicz, Z., et al. (2010).  Salivary glands -- ‘an unisex organ’? Oral Dis, 16(7), 577-85.
  16. Pomari, E., Nardi, A., Fiore, C., et al. (2009).  Transcriptional control of human organic anion transporting polypeptide 2B1 gene.  J Steroid Biochem Mol Biol, 115(3-5), 146-52.
  17. Jezova, D., Hlavacova, N. (2008). Endocrine factors in stress and psychiatric disorders: Focus on anxiety and salivary steroids.  Ann N Y Acad Sci, 1148, 495-503.

​The effects of freeze thaw on most biological measures, regardless of biospecimen type, can be dramatic. Analytes in oral fluid are not distinct or different in this way. As a general rule, multiple freeze-thaws should be avoided.  The most practical way to address this concern is by aliqouting samples after collection. Some analytes are more resistant to freeze thaw than others. We recommend that investigators consult the literature for the analytes of interest. If there is freeze thaw data for a specific biological measure in traditional biospecimens, it is reasonable to assume this would also be true for saliva.


​No, but the literature is rather extensive on this subject for several salivary analytes; especially for salivary alpha-amylase and cortisol. We do not track that information internally. 


​With modern search tools online, we no longer maintain records of this type. We suggest that you use Pubmed or Psychlit to search the literature or you can Ask An Expert and we will be happy to assist you in your search.


​If the analyte you are interested in is not noted in our website, please contact Dr. Granger at [email protected] to find out what developments are in the pipeline.


​Salimetrics does not release products for sale if the quality control (QC) testing shows any issues. Here are some probable causes:

1) Can timing of adding reagents be off?  For instance with a multichannel you can pipette the conjugate and TMB so many times before you refill, but you can pipette the stop twice as fast because it is a smaller volume.  If you do this, it shortens the time the bottom rows have with TMB.

2) Can your washer be uneven and sheering off antibody in the bottom corner?  Aspirate and check the amount of fluid left.  It should be even in all wells and no wells should be completely dry.

3) Are you mixing faster than recommended?  Or slower?  

4) Are all reagents completely at room temperature?  A bottle of assay diluent takes 2 hours to come to RT.  You can pour some off into a smaller tube to warm up quicker for the zero and nsb.

5) Are you leaving the plate come to room temperature BEFORE opening the bag? (Otherwise moisture due to humidity may form in the wells and this is particularly a problem in this high humidity weather)

6) Are your multichannel pipettes dispensing the same amount each time reliably?  We discard the first and last dispenses as they are not as reliable.

7) Are you incubating with TMB in the dark?  (We no longer recommend aluminum foil.)

8) Are you testing one plate at a time?  For example, do not put the standards on 5 plates then go back and fill in with samples. This delays the addition of conjugate to the plates.

9) Clean your plate reader filter. Dust from the lab can collect on the filter.

10) Are you adding assay diluent to the zero in sequence after the standards and not the last thing?

11) Never put the multichannel pipette tips into the wells as you can drag down standard from the wells above it causing lower readings in other wells.

12) Thoroughly blot all wells just before adding TMB but do not let the plate dry out.