Estradiol

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Overview

Serum-Saliva Correlation 0.80
Sensitivity 0.1 pg/mL
Sample Test Volume 100 μL
Recommended Collection Volume 225 μL*
Special Considerations No
Collection Protocol Download PDF

Description


  1. Abraham, G.E. (1975). The applications of steroid radioimmunoassay to gynecologic endocrinology. In: Taymor, M.L. and Green, T.H. (eds.): Progress in gynecology, Vol. 1, 111-144. New York: Grune and Stratton.
  2. Faiman, C., Winter, S.D., & Reyes, F.I. (1976). Patterns of gonadotropins and gonadal steroids throughout life. Clin Obstet Gynecol, 3(3), 467-483.
  3. Kirschner, M.A., Schneider, G., Ertel, N.H., Worton, E. (1982).  Obesity, androgens, estrogens, and cancer risk.  Cancer Res, 42 (8 suppl), 3281s-3285s.
  4. Labrie, F., Bélanger, A., Cusan, L., Candas, B. (1997).  Physiological changes in dehydroepiandrosterone are not reflected by serum levels of active androgens and estrogens but of their metabolites: Intracrinology.  J Clin Endocrinol Metab, 82(8), 2403-9.
  5. Lipson, S.F., & Ellison, P.T. (1996). Comparison of salivary steroid profiles in naturally occurring conception and non-conception cycles. Hum Reprod, 11(10), 2090-96.
  6. Choe, J.K., Khan-Dawood, F.S., Dawood, M.Y. (1982). Progesterone and estradiol in saliva and plasma during the menstrual cycle. Am J Obstet Gynecol, 146, 557-62.
  7. Bao, A.-M., Liu, R.-Y., van Someren, E.J., et al. (2003).  Diurnal rhythm of free estradiol during the menstrual cycle.  Eur J Endocrinol, 148(2), 227-32.
  8. Chang, R.J., Plouffe Jr., L. Schaffer, K.  Physiology of the menopause. In: Comprehensive management of menopause, Lorrain, J., Flouffe Jr., L., Ravnikar, V., Speroff, L., Watts, N., eds.  New York: Springer, 1993.
  9. Reed, M.J., Lai, L.C., Owen, A.M., et al. (1990).  Effect of treatment with 4-hydroxyandrostenedione on the peripheral conversion of androstenedione to estrone and in vitro tumor aromatase activity in postmenopausal women with breast cancer.  Cancer Res, 50(1), 193-96.
  10. Shirtcliff, E.A., Dahl, R.E., Pollak, S.D. (2009). Pubertal development: Correspondence between hormonal and physical development.  Child Dev, 80(2), 327-37.
  11. Simpson, E.R. (2000). Role of aromatase in sex steroid action.  J Mol Endocrinol, 25(2), 149-56.
  12. Winters, S.J., Troen, P. (1986).  Testosterone and estradiol are co-secreted episodically by the human testis.  J Clin Invest, 78(4), 870-73.
  13. Nankin, H.R., Pinto, R., Fan, D.-F., Troen, P. (1975).  Daytime titers of testosterone, LH, estrone, estradiol, and testosterone-binding protein: Acute effects of LH and LH-releasing hormone in men.  J Clin Endocrinol Metab, 41(2), 271-81.
  14. Ouyang, P., Michos, E.D., Kara, R.H. (2006). Hormone replacement therapy and the cardiovascular system: Lessons learned and unanswered questions.  J Am College Cardiol, 47(9), 1741-53.
  15. McCarthy, M.M. (2008). Estradiol and the developing brain. Physiol Rev, 88(1), 91-134.
  16. Balthazart, J., Cornil, C.A., Taziaux, M., et al. (2006). Rapid changes in production and behavioral action of estrogens. Neuroscience, 138(3), 783-91.
  17. Karpuzoglu, E., Ahmed, S.A. (2006).  Estrogen regulation of nitric oxide and inducible nitric oxide synthase (iNOS) in immune cells: Implications for immunity, autoimmune diseases, and apoptosis.  Nitric Oxide, 15(3), 177-86.
  18. Colditz, G. A. 1998.  Relationship between estrogen levels, use of hormone replacement therapy, and breast cancer. J Natl Cancer Inst, 90(11), 814-23.
  19. Lépine, J., Audet-Walsh, E., Grégoire, J., et al. (2010)  Circulating estrogens in endometrial cancer cases and their relationship with tissular expression of key estrogen biosynthesis and metabolic pathways.  J Clin Endocinol Metab, 95(6), 2689-98.
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  22. Choe, J.K., Khan-Dawood, F.S., & Dawood, M.Y. (1983). Progesterone and estradiol in saliva and plasma during the menstrual cycle. Am J Obstet Gynecol, 147(5), 557-62.
  23. Shirtcliff, E.A., Granger, D.A., Schwartz, E.B., et al. (2000). Assessing estradiol in biobehavioral studies using saliva and blood spots: Simple radioimmunoassay protocols, reliability, and comparative validity. Horm Behav, 38(2), 137-47.
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​The effects of freeze thaw on most biological measures, regardless of biospecimen type, can be dramatic. Analytes in oral fluid are not distinct or different in this way. As a general rule, multiple freeze-thaws should be avoided.  The most practical way to address this concern is by aliqouting samples after collection. Some analytes are more resistant to freeze thaw than others. We recommend that investigators consult the literature for the analytes of interest. If there is freeze thaw data for a specific biological measure in traditional biospecimens, it is reasonable to assume this would also be true for saliva.

 

​No, but the literature is rather extensive on this subject for several salivary analytes; especially for salivary alpha-amylase and cortisol. We do not track that information internally. 

 

​With modern search tools online, we no longer maintain records of this type. We suggest that you use Pubmed or Psychlit to search the literature or you can Ask An Expert and we will be happy to assist you in your search.

 

​If the analyte you are interested in is not noted in our website, please contact Dr. Granger at [email protected] to find out what developments are in the pipeline.

 

​Salimetrics does not release products for sale if the quality control (QC) testing shows any issues. Here are some probable causes:

1) Can timing of adding reagents be off?  For instance with a multichannel you can pipette the conjugate and TMB so many times before you refill, but you can pipette the stop twice as fast because it is a smaller volume.  If you do this, it shortens the time the bottom rows have with TMB.

2) Can your washer be uneven and sheering off antibody in the bottom corner?  Aspirate and check the amount of fluid left.  It should be even in all wells and no wells should be completely dry.

3) Are you mixing faster than recommended?  Or slower?  

4) Are all reagents completely at room temperature?  A bottle of assay diluent takes 2 hours to come to RT.  You can pour some off into a smaller tube to warm up quicker for the zero and nsb.

5) Are you leaving the plate come to room temperature BEFORE opening the bag? (Otherwise moisture due to humidity may form in the wells and this is particularly a problem in this high humidity weather)

6) Are your multichannel pipettes dispensing the same amount each time reliably?  We discard the first and last dispenses as they are not as reliable.

7) Are you incubating with TMB in the dark?  (We no longer recommend aluminum foil.)

8) Are you testing one plate at a time?  For example, do not put the standards on 5 plates then go back and fill in with samples. This delays the addition of conjugate to the plates.

9) Clean your plate reader filter. Dust from the lab can collect on the filter.

10) Are you adding assay diluent to the zero in sequence after the standards and not the last thing?

11) Never put the multichannel pipette tips into the wells as you can drag down standard from the wells above it causing lower readings in other wells.

12) Thoroughly blot all wells just before adding TMB but do not let the plate dry out.