DNA: Oxytocin Receptor - OXTR

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Overview

Methodology

Genomic DNA, purified using silica based...learn more

Recommended Collection Method

SOS (preferred), SIS, SCS or 500 uL Passive Drool

Yield

2-5 ug genomic DNA (?)

Special Considerations

Methods Paper: Assessing genetic polymorphisms using DNA extracted from cells present in saliva samples.

Collection Protocol Download PDF

Description


  1. Lerer, E. et al. (2008). Association between the oxytocin receptor (OXTR) gene and autism: relationship to Vineland Adaptive Behavior Scales and cognition. Molecular Psychiatry. 13, 980-988.
  2. Skuse, D. et al. (2014). Common polymorphism in the oxytocin receptor gene (OXTR) is associated with human social recognition skills. PNAS. 111(5), 1987-1992.
  3. Rodrigues, S. et al. (2009). Oxytocin receptor genetic variation relates to empathy and stress reactivity in humans. PNAS. 106(50), 21437-21441.
  4. Spahire-Bernstein, S. et al. (2011). Oxytocin receptor gene (OXTR) is related to psychological resources. PNAS. 108(37), 15118 – 15122.

​No, the fluid in the Oragene device will dilute the saliva by an unknown amount and also has the potential to interfere with EIA, thus impeding the accurate measurement of analytes.

 

​Yes, saliva collected for DNA analysis using SalivaBio or Salimetrics collection devices can be used for other analytes as well. DNA in whole saliva is obtained from the cell pellet after centrifugation.  The supernatant can be used for testing other analytes.  DNA is collected from the actual SalivaBio swabs after centrifugation, so the saliva in the swab storage tube can be used testing other analytes.  Salimetrics recommends collecting the DNA material before testing for other analytes but it still possible to get the DNA sample after testing for other analytes provided that care is taken to prevent cross contamination during the testing phase. See this publication for more information;

Zsofia Nemoda, Maria Horvat-Gordon, Christine K Fortunato, Emilie K Beltzer, Jessica L Scholl and Douglas A Granger;Assessing genetic polymorphisms using DNA extracted from cells present in saliva samples;BMC Medical Research Methodology 2011, 11:170 doi:10.1186/1471-2288-11-170.