How To's

The Latest Saliva Research Advice and Advisories 

As technology emerges we may discover new applications and limitations of salivary measures; but you can operate on the cutting edge with the most recent advice from Salimetrics. Below you will find the Saliva Collection Handbook, which features the most relevant saliva collection and handling information, as well as a number of biomarker-specific articles.

Select your biomarkers of interest to determine which articles are most relevant to your research.



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Aliquoting Tips - Splitting Samples

Length: 6:19
Description: In this video we demonstrate how to split saliva samples through aliquoting. We demonstrate proper technicque and also review workspace prep and sample organization.



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Saliva Collection Methods and Techniques Handbook

Length: 18 Pages
Description: Learn the proper techniques and methods for saliva collection, handling, storage, and shipping with this step-by-step guide. If you’re collecting saliva for biomarker or analyte concentration analysis by ELISA, we’ve developed a better guide to provide you better results in your salivary testing…

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Handling Multiple Salivary Analytes and Order of Assay

Description: Many bio-behavioral studies now include measurements of multiple salivary biomarkers. Some analytes such as cortisol and alpha-amylase are fairly stable at room temperature, and they present few problems for handling in the laboratory. The sex steroids (estrogens, progesterone, and testosterone) and…

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Interpreting Differences in EIA Results

Description: Occasionally, differences in EIA results are observed from what is expected. In most cases, these differences relate to one or more factors: kit storage conditions, faulty laboratory equipment, technician error, or other factors that are likely to be causing the poor performance. If kit users experience…

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Collecting and Handling Saliva for Analysis of Novel Protein and Peptide Markers

Description: Saliva contains numerous protein-degrading enzymes, and levels of these enzymes are elevated in individuals with periodontal disease. (Watanabe et al., 1981; Ingman et al., 1993; Uitto et al., 1990) It is thought that many of these enzymes originate from inflammatory cells or microbes present in the…

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Guidelines for Interpreting Cotinine Levels

Length: 4 Pages
Description: We recommend the following guidelines when interpreting results from the Salimetrics Cotinine EIA

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Tips for Running Salimetrics Assays

Description: To obtain consistent results, it is important to always use the same methods, equipment, timing, etc., when testing saliva samples. Salimetrics recommends that all saliva samples be frozen, thawed and centrifuged before use in our assays.  (Freezing precipitates particulate matter and mucins.) …

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Collecting and Handling Saliva Samples for Cytokine Analysis

Description: As was noted in the Spit Tip from our earlier Spit Report devoted to salivary CRP (April 2011), limited information may exist on the best ways to collect and handle saliva for the analysis of many novel biomarkers. In the case of salivary cytokines such as IL-1ß, IL-6, and TNF-a, however, the modest…

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Smoking Definitions for Salivary Cotinine Researchers

Description: One of the difficulties encountered in identifying smokers by self report is that there are many different ideas of what constitutes smoking behavior. For example, when asked about their smoking status, light or intermittent smokers will often classify themselves as nonsmokers. In order to maintain consistency…

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Collecting and Handling Saliva Samples for Analysis of Novel Salivary Biomarkers

Description: Ongoing research is identifying many molecules in saliva that are of interest as biomarkers for various research and diagnostic applications. Limited information exists, however, on the best ways to collect and handle saliva for the analysis of many of these molecules. In contrast to better-known salivary…

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Inter- and Intra-Assay Coefficients of Variability

Description: In order to express the precision, or repeatability, of immunoassay test results, researchers in the social and behavioral sciences typically report two measures of the Coefficient of Variability (CV) in their publications.

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Saliva Collection from Infants and Small Children

Description: Studies have reported that some older preschoolers are able to donate saliva samples by spitting or drooling, (1,2) but researchers have generally found that using some sort of absorbent device is the best way to collect saliva from young children. Parents often have a better chance of getting the child…

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Collecting and Handling Saliva for DNA Analysis

Description:   Request Quote     Ask An Expert    Research SNPs   DNA in Saliva The DNA in saliva originates from cells that are shed from the inner linings of the mouth and from white blood cells. These DNA-containing cells are collected, and the DNA is then extracted…

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Importance of Mouth Location During Saliva Collection

Length: 7 Pages
Description: Concentrations of some salivary analytes vary depending on the location in the mouth.

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Correcting Salivary a-Amylase Activity for Flow Rate

Description: Although a recent paper* has stated that salivary α-amylase (sAA) concentrations are not affected by saliva flow rates, Salimetrics is aware that this matter is currently being re-examined.  Until the question of flow-rate dependency for sAA is definitively settled, Salimetrics is advising…